Human Cytomegalovirus (CMV) infection is one of the most common viral infection globally. CMV seroprevalence was found to be 40% to 100% depending on the socioeconomic conditions. CMV infection is commonly asymptomatic, it remains latent throughout life and may reactivate. Individuals at increased risk of CMV infection include neonates, pregnant women and immuno-compromised adults. Congenital CMV infection from mother to fetus during pregnancy could lead to a series of pregnancy complications including fetal anomaly, mental retardation, In-Utero Growth Retardation (IUGR), visual impairment and neurosensory hearing loss. Early diagnosis of congenital CMV infection is recommended and antibody test cannot be used for the diagnosis of congenital CMV infection. Diagnosis can only be made if the CMV is found in the infant's blood, urine and saliva sample within one week of birth. Therefore, a rapid, sensitive and reliable CMV detection test should be adopted.